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KMID : 0370219960400010052
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Yakhak Hoeji
1996 Volume.40 No. 1 p.52 ~ p.58
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Screening for Inhibitory Effect of Solvent Fractions Prepared from Herbal Drugs on CCl4-induced Cytotoxicity in Primary Cultured Rat Hepatocytes and Evaluation of Antihepatotoxicity in Vivo
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김영숙/Kim YS
경종수/박기현/Kyung JS/Park KH
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Abstract
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Solvent fractions were prepared from traditional herbal drugs which of methanol extracts inhibited CCl4-induced cytotoxicity in primary cultured rat hepatocytes and continuously assayed their effects. Ethylacetate and n-buthanol fractions from Cibotii Rhizoma and chloroform fraction from Gelatina Nigra inhibited the release of LDH and GPT from CCl4-treated hepatocytes, respectively. Water fraction (WAR) among solvent fractions from Astragali Radix showed the most potent inhibitory effect on the release of GOT or GPT by treatment with CCl4. All of solvent fractions prepared from Eucommiae Cortex had no effect on CCl4-induced cytotoxicity. Chloroform and ethylacetate fractions from Rehmanniae Radix Preparata increased the release of GPT from CCl4-treated hepatocytes. n-Hexan, chloroform or ethylacetate fraction from 5 herbal drugs increased the release of LDH, GOT or GPT from normal hepatocytes at the dose of 1.Omg/ml. Administration of WAR suppressed the elevation of GOT, ALP activities and MDA contents in the serum as well as in the liver tissue of CCl4-intoxicated rats. Based on these results, isolation of antihepatotoxic substances from WAR is under the process.
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